Amisha Patel ne apni career ki shuruat 1999 mein Bollywood film "Neeha" se ki thi, lekin unhein pehchaan 2000 mein aayi film "Kahne Do" se mili. Iske baad unhone kai safal filmein ki, jinmein se kuchh pramukh hain: "Chal Mere Bhai", "Aks", "Kya Love Story Hai", "Zindagi Na Milegi Dobara", aur "Bajirao Mastani".